| NMC Nayanakantha*, BR Singh and Anil Kumar
Rubber Research Institute of Sri Lanka, Dartonfield, Agalawatta, Sri Lanka
Abstrsact
An efficient micropropagation protocol was developed using lateral shoot explants of Aloe vera. Both shoot induction and elongation were better on MS medium supplemented with 4mg/l BAP + 0.2mg/l NAA + 1g/l PVP. All cultures showed shoot regeneration in this medium with 16 shoots/explant. Addition of 1g/l PVP was ineffective for controlling phenolic browning of explants and culture discoloration. However, more adventitious buds (21.5 shoots/explant) developed on MS medium supplemented with 4mg/l BAP + 0.2mg/l NAA + 1g/l PVP+10mg/l citric acid+0.5g/l activated charcoal. Browning of explants was minimized in this medium and elongation of micro-shoots and the growth of the plantlets were also better. Further elongation and rooting of micro-shoots were obtained when sub-cultured on to MS basal medium containing 0.5g/l activated charcoal and 100% of the survival of rooted plantlets was observed after acclimatization. Therefore, the above protocol could be effectively used in rapid micropropagation of elite plants of Aloe vera.
Key words: Aloe vera, Phenolic browning, Micropropagation, Culture media, Growth hormones
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